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KMID : 0381219880200020150
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Journal of RIMSK
1988 Volume.20 No. 2 p.150 ~ p.154
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Study on the Proportions of T,B Lymphocytes and T Lymphocyte Subpopulations in Normal Neonates
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Chae Soo-Ane
Shim Tae-Sub
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Abstract
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The quantitation of TB lymphocytes and T lymphocyte sub populations is important in the diagnosis and the prognosis of malignant neoplasm’s, auto immune diseases, and immunodeficient diseases.
The development of specific monoclonal antibodies to antigens present on all peripheral T cells (OKT3), helper/inducer (OKT4), and cytotoxic/suppressor (OKT8) sub populations provides a simple means to dissect the immune cell populations.
Although the study on the proportions of TB lymphocyte sub populations in adult healthy Koreans was published, there has been no published study in normal neonates.
The authors attempted to evaluate the distribution of T,B lymphocyte sub populations in normal neonates. Blood from 18 healthy neonates and 17 normal adult control group were collected and lymphocyte separation was prepared by Boyms technique and were assayed for the evaluation of total lymphocytes(OKT3), helper/inducer(OKT4), cytotoxic/suppressor(OKT8) sub populations and pan B-lymphocytes (OKB7).
The results were as follows;
1) In normal neonates, the value of OKT3 55.5±15.90%, OKT4 37.8 ±7.25%, OKB7 21.8±10.16%, and T4/T8 ratio 1.5 ±0.64 were significantly lower than healthy adult control group that showed OKT3 66.5±10.60%, OKT4 47.2 ±15.02%, OKB7 32.8 ±10.75%, and T4/T8 ratio 1.87±0.47(P>0.05). The value of OKT8 in normal neonates, 27.8 ±8.32%, was higher than healthy adult control group, 25.3±7.30%.
2) In normal neonates, number of total lymphocytes 5,131±1,5841μl; OKT3, 3,021±1,165/1; OKT4,1,901±636/1P; and OKT8, 1,437±762/μl were statistically higher than normal adult control group that showed total lymphocytes, 3,304±922 μl; OKT3, 2,180±717/μl; OKT4, 1,534±564/μl; OKT8, 793±283/μl. Number of OKB7 in normal neonates was not significantly different from that of normal adult control group. According to the above results, the proportions of T,B lymphochtes and T lymphocyte sub populations in normal neonates were significantly different from that normal adult control group. These findings may be the consequence of the presence of immature Tcells in neonatal peripheral blood.
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KEYWORD
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